Individual Study / MeaTIc

Faecal microbiome as determinant of the effect of diet on colorectal-cancer risk: comparison of meat based versus pesco-vegetarian diets.
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Faecal microbiome as determinant of the effect of diet on colorectal-cancer risk: comparison of meat based versus pesco-vegetarian diets.

Networks -
Datasets -
Variables -
Start Year
2020
End Year
2021
Funding
Italian Ministry Research University
Supplementary Information
Genotoxic activity of faecal water (primary outcome). Faecal Microbioma profiles, Faecal Targeted Metabolomics profiles (SCFAs, Aminoacids and Secondary bile acids), Mutagenicity of faecal water , Toxicity of faecal water, Markers of global peroxidation of faecal water (TBARs), Markers of specific peroxidation of omega 3 and omega 6 PUFA in faecal water (HNE-HE), Blood Lipid profile: total cholesterol, low density lipoprotein cholesterol (LDL), high density lipoprotein cholesterol (HDL), triglycerides, Glycemic profile: glucose, insulin, glycated hemoglobin (HbA1C), HOMA index, hs-CRP (high-sensitivity C-reactive protein). Circulating levels of inflammatory cytokines.Urinary biomarker: 1,4-dihydroxynonane mercapturic acid (DHN-MA) is the major urinary metabolite of 4-
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Members

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Investigators Contacts
  • Prof. Giovanna Caderni
    University of Florence, CNR-IBBA
  • Dr. Carlotta De Filippo
    CNR-IBBA
  • Dr. Jildau Bowman
    TNO
  • Prof. Lisa Giovannelli
    University of Florence
  • Prof. Francesco Sofi
    University of Florence
  • Prof. Giovanna Caderni
    University of Florence, CNR-IBBA
  • Dr. Carlotta De Filippo
    CNR-IBBA

Design

Study design
Other : control arm of intervention study
Follow Up
No

Marker Paper

Fecal microbiome as determinant of the effect of diet on colorectal cancer risk: comparison of meat-based versus pesco-vegetarian diets (the MeaTIc study).

PUBMED 31815647

Recruitment

Sources of Recruitment
  • Individuals

Number of participants

Number of participants
113
Number of participants with biosamples
Supplementary Information
Recruitment through clinics and through advertisement

Timeline

meatic

Selection Criteria
Minimum age
18
Maximum age
50
Newborns
Twins
Countries
  • Italy
Ethnic Origin
Health Status
  • healthy volounteers undergoing an intervention trial

Recruitment

Sources of recruitment
  • General population
General Population
  • Volunteer enrolment

Number of participants

Number of participants
113
Number of participants with biosamples
Data Collection Event
Start Date
2020
End Date
2021

Measurements & methods

Dietary intake

yes/no
  • Information collected on habitual food intake collected
  • Information collected on type of diet (vegetarian/vegan/omnivore)
  • Special dietary aspects collected (e.g. supplements, probiotics, prebiotics and synbiotics)
Methods
  • FFQ
  • Food records
  • Other methods
FFQ
Questionnaire name/set of questions
Italian medium-FFQ
Type of questionnaire
Semi-quantitative
Administration method
Self-reported
Administration type
Paper-based
Number of food items
27
Reference period
year
Frequency categories
varying
Portion size estimation
Standard portion sizes without aids
Reproducibility
no
Instrument validity
no
Instrument development
already existing instrument (medium-FFQ)
Instrument reference
https://pubmed.ncbi.nlm.nih.gov/25830946/
Food records
Administration method
Self-reported
Administration type
Paper-based
Number of recorded days
2-7
Instrument validity
no
Instrument development
already existing instrument
Instrument reference
3-day weighted dietary record (WDR)
Other methods
Other methods
Medi-Lite adherence score
Instrument(s) access
https://pubmed.ncbi.nlm.nih.gov/28276908/

Alcohol consumption

Information collected
quantity and frequency of consumption of alcoholic drinks
Questionnaire name/set of questions
medium-FFQ, Medi-Lite adherence score
Administration method
Self-reported
Administration type
Paper-based

Tobacco consumption

Information collected
consumption (yes/no)
Questionnaire name/set of questions
Set of questions
Administration method
Face-to-face iinterview
Administration type
Paper-based

Physical activity

Information collected
frequency, duration and types of PA
Questionnaire name/set of questions
set of questions
Administration method
Face-to-face interview
Domains covered
leisure, occupation

Anthropometric measurements

Weight/height
Measured
Waist/hip circumference
no
Other measurements
body composition (BIA)

Sociodemographic information

Information collected
sex, age, residence, country of birth, country of citizenship at time of data collection
Questionnaire name/set of questions
set of questions
Administration method
Face-to-face interview

Medication

Information collected
drug name
Questionnaire name/set of questions
set of questions
Antibiotics
yes
Administration method
Face-to-face interview

Health status

Information collected
disease diagnosis
Questionnaire name/set of questions
set of questions
Administration method
Face-to-face interview
Measurements
various

Biological samples and laboratory measurements

Samples collected
blood, urine, stool
Number of samples
113

Biomarkers

Lipids

Measurement(s)
HDL, LDL, cholesterol
Medium
Serum

Glucose

Measurement(s)
glucose
Medium
serum

Other biomarkers

Measurement(s)
Folate, vitamin B12, iron, ferritin, triglycerides, AST, ALT, uric acid, urea, creatinine, pro- and anti-inflammatory profile, cytokines, urinary biomarkers of lipid peroxidation, Markers of global peroxidation of fecal water (TBARs) and specific peroxidation of omega-3 and omega-6 PUFA in fecal water
Medium
other

Metabolomics

Measurement(s)
SCFAs, Aminoacids, Bile acids, Fecal water untargeted metabolomics
Medium
other
Untargeted approach
no
Targeted groups
lipids, aminoacids, secondary bile acids

Microbiome

Microbial material
DNA, 16S rRNA (V3 and V4 hypervariable regions), Fecal microbiota profiles
Number of samples/number of individuals
113
Library source and library selection
Metagenome, serum and urine metabolome
Medium/tissue
faeces
Sampling collection method
samples on dry ice
Time spent in room temperature
none
Sequencing platform
Illumina MiSeq
Preservation method
Immediate -20, dry ice/-80 within 4-24h
Information on type of meal last eaten prior to sample extraction available
no