Individual Study / LISA

Influence of Life-style related factors on the development of the Immune System and Allergies in East and West Germany
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Influence of Life-style related factors on the development of the Immune System and Allergies in East and West Germany

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Datasets -
Variables -
Start Year
1997
Funding
BMBF, IUF, HMGU, EU
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Investigators Contacts
  • Dr. Marie Standl
    HMGU

Design

Study design
Cohort
Follow Up
yes, 20 years

Recruitment

Sources of Recruitment
  • Individuals

Number of participants

Number of participants
3,094
Number of participants with biosamples

Timeline

LISA

Selection Criteria
Minimum age
0
Newborns
Twins
International/National/Regional (region name)
Regional (Munich, Wesel, Leipzig, Bad Honnef); Microbiome data only available from Munich subset
Ethnic Origin
Health Status

Recruitment

Sources of recruitment
  • General population

Number of participants

Number of participants
3,094
Number of participants with biosamples
Data Collection Event
Start Date
1997

Measurements & methods

Dietary intake

yes/no
  • Information collected on habitual food intake collected
  • Information collected on type of diet (vegetarian/vegan/omnivore)
  • Special dietary aspects collected (e.g. supplements, probiotics, prebiotics and synbiotics)
Methods
  • FFQ
FFQ
Questionnaire name/set of questions
FFQ (at 10, 15 and 20 years)
Type of questionnaire
Semi-quantitative
Administration method
Self-reported
Number of food items
80
Reference period
Year
Frequency categories
9
Portion size estimation
Standard portion sizes with aids such as pictures, models, standard household measure, utensils, etc.
Reproducibility
No
Instrument validity
Yes
Instrument development
Newly-developed
Instrument reference
Stiegler, P., Sausenthaler, S., Buyken, A., Rzehak, P., Czech, D., Linseisen, J., . . . Heinrich, J. (2010). A new FFQ designed to measure the intake of fatty acids and antioxidants in children. Public Health Nutrition, 13(1), 38-46. doi:10.1017/S1368980009005813

Alcohol consumption

Information collected
Frequency of consumption of alcoholic drinks
Questionnaire name/set of questions
Set of questions
Administration method
Self-reported

Tobacco consumption

Information collected
Smoking status and number of cigarrettes per day
Questionnaire name/set of questions
Set of questions
Administration method
Self-reported

Physical activity

Information collected
Frequency, duration and types of PA
Questionnaire name/set of questions
Set of questions
Administration method
Self-reported
Domains covered
Total light / moderate / vigorous PA

Sedentary behaviours

Information collected
Time spent sedentary
Questionnaire name/set of questions
Set of questions
Administration method
Self-reported

Anthropometric measurements

Weight/height
Self reported / measured
Other measurements
body composition (BIA)

Sociodemographic information

Information collected
Sex, age, residence, education, parental education, parental income
Questionnaire name/set of questions
Set of questions
Administration method
Self-reported

Medication

Information collected
Drug names and the pharmaceutical identification numbers (PZNs)
Questionnaire name/set of questions
Set of questions
Administration method
Self-reported

Health status

Information collected
Disease diagnosis
Questionnaire name/set of questions
Set of questions

Biological samples and laboratory measurements

Samples collected
Blood
Number of samples
1088

Biomarkers

Lipids

Measurement(s)
LDL, HDL, cholesterol, TAG
Medium
Serum

Glucose

Measurement(s)
Glucose (mmol/L), insulin (pmol/L), at 15 years
Medium
Blood (glucose), serum (insulin), at 15 years

Inflammation markers

Measurement(s)
Hs-CRP (10y and 15y), IL-6 (only 10y follow-up)
Medium
Serum

Adiposity biomarkers

Measurement(s)
Adiponectin, leptin (only 10y follow-up)
Medium
Serum

Other biomarkers

Measurement(s)
Yes, various
Medium
Serum

Metabolomics

Measurement(s)
LC-MS/MS, subset at 10 and 15 year
Medium
Serum
Untargeted approach
no
Targeted groups
Amino acids, polar lipids, NEFA, other carboxylic acids (TCA)

Microbiome

Microbial material
16S (V3–V4), at 6 years in Munich subset
Number of samples/number of individuals
166
Medium/tissue
Faeces
Time spent in room temperature
Unclear
Sequencing platform
Illumina MiSeq
Read length/depth
reference: https://www.nature.com/articles/s41598-019-49160-7
Further details
reference: https://www.nature.com/articles/s41598-019-49160-7
Preservation method
Delivery from participant's house to study center with taxi, then -80°C until analysis
Preservation buffer
Native
Information on type of meal last eaten prior to sample extraction available
no