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ErNst
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ErNst

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Start Year
2018
End Year
2018
Funding
BMEL, German federal ministry of food and agriculture
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Investigators Contacts
  • Dr. Andreas Dötsch
    Max Rubner-Institut
  • Dr. Andreas Dötsch
    Max Rubner-Institut

Design

Study design
Cross-sectional
Follow Up
no follow up

Marker Paper

Dötsch et al. (in preparation): "Cross-sectional Human Observational Study of Energy and Nutrient Intake and the Intestinal Microbiome"

Recruitment

Sources of Recruitment
  • Individuals

Number of participants

Number of participants
117
Number of participants with biosamples
106
Supplementary Information
Total number of participants was 117. Of these, full sets of biosamples (stool) and FFQs were obtained from 106 of these (53 M 53 F).

Timeline

ernst

Selection Criteria
Minimum age
18
Maximum age
79
Newborns
Twins
Countries
  • Germany
International/National/Regional (region name)
Europe
Ethnic Origin
  • not assessed
Health Status
  • healthy (without any known or apparent acute or chronic illness/disease)

Recruitment

Sources of recruitment
  • General population
  • Other : Recruitment through Study Center via database for study participants (through mail and E-Mail), leaflets, homepage etc.

Number of participants

Number of participants
117
Number of participants with biosamples
106
Supplementary Information
Total number of participants was 117. Of these, full sets of biosamples (stool) and FFQs were obtained from 106 of these (53 M 53 F).
Data Collection Event
Start Date
2018-10
End Date
2018-12

Measurements & methods

Dietary intake

yes/no
  • Information collected on habitual food intake collected
  • Information collected on type of diet (vegetarian/vegan/omnivore)
Methods
  • 24-hour dietary recall
  • FFQ
24-hour dietary recall
Administration method
Face-to-face interview
Administration type
Web/computer-based
Number of recalls
1
Portion size estimation
Standard portion sizes with aids such as pictures, models, standard household measure, utensils, etc.
Instrument validity
Testing validity of the updated instrument was one of the study aims.
Instrument development
Updated version of GloboDiet
Instrument reference
Slimani et al. (1999), doi:10.1016/s0169-2607(98)00088-1; Slimani et al. (2011), doi: 10.1038/ejcn.2011.83
FFQ
Questionnaire name/set of questions
DEGS FFQ/RKI (modified version)
Type of questionnaire
Semi-quantitative
Administration method
Self-reported
Administration type
Paper-based
Number of food items
48
Reference period
1 month (30 days)
Frequency categories
11
Portion size estimation
Standard portion sizes without aids
Reproducibility
no
Instrument validity
no
Instrument development
Adapted from DEGS FFQ/RKI
Instrument reference
Haftenberger et al., Nutrition Journal volume 9, Article number: 36 (2010); doi: 10.1186/1475-2891-9-36.

Alcohol consumption

Information collected
Categorical (no, occassionally, regularly)
Questionnaire name/set of questions
Single question
Administration method
Self-reported
Administration type
Paper-based

Tobacco consumption

Information collected
Frequency of smoking cigarettes and other tobacco products; history (total years of smoking)
Questionnaire name/set of questions
Set of questions
Administration method
Self-reported
Administration type
Paper-based

Physical activity

Device type
Accelerometer (ActiHeart/ CamNtech)
Device model
ActiHeart (Software version: 4.0.131)
Measurement period
24h
Measurement position
Chest lead: one electrode is adhered at V1 or V2 (4th intercostals) and the second electrode is placed approximately 10cm away on the left side at V4 or V5

Anthropometric measurements

Weight/height
Measured
Other measurements
Body composition (BIA)

Sociodemographic information

Information collected
Sex, age
Questionnaire name/set of questions
Set of questions
Administration method
Face-to-face interview
Administration type
Web/computer-based

Medication

Information collected
Drug dosage and name
Questionnaire name/set of questions
Set of questions
Antibiotics
Yes (treatment within last 6 months)
Administration method
Self-reported
Administration type
Paper-based

Health status

Information collected
Medical history
Questionnaire name/set of questions
Various
Administration method
Face-to-face interview

Biological samples and laboratory measurements

Samples collected
Urine (24h), stool
Number of samples
109 (urine), 106 (stool)

Biomarkers

Other biomarkers

Measurement(s)
Sodium, potassium, nitrogen, osmolality, creatinine
Medium
Urine

Microbiome

Microbial material
DNA
Number of samples/number of individuals
212
Library source and library selection
16S Amplicons (V4 region)
Medium/tissue
Faeces
Sampling collection method
Frozen (-20°)
Time spent in room temperature
Short (immediatey freezing after collection)
Sequencing platform
MiSeq
Read length/depth
2x301/20000 - ca. 100000 on average per sample
Further details
Amplicon sequencing
Preservation method
Immediate -20, transport on cooling packages, mid-/long-term storage at -80 until aliquotation and DNA isolation
Preservation buffer
None
Information on type of meal last eaten prior to sample extraction available
no