Elderly microbiome study (Italian cohort from NU-AGE)
Networks
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Datasets
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Variables
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* to counteract the physical/cognitive decline by a whole diet intervention;
* to assess the effect of a 65+ food pyramid using biomarkers;
* to identify cellular/molecular targets/mechanisms responsible for the whole diet effect;
* to perform genetic and epigenetic studies to assess the role of individual variability in the response to diet;
* to adopt an integrative comprehensive approach to analyse the whole data set.
- Start Year
- 2012
- End Year
- 2015
- Funding
- NU-AGE was funded under FP7-KBBE (2011-2016), project coordinator: Claudio Franceschi
| Investigators | Contacts |
|---|---|
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Design
- Study design
- Other
Marker Paper
https://www.tandfonline.com/doi/full/10.1080/19490976.2021.1880221 DOI: 10.1080/19490976.2021.1880221
PUBMED 33557667
Recruitment
- Sources of Recruitment
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- Individuals
Number of participants
- Number of participants
- 201
- Number of participants with biosamples
- Supplementary Information
- 101 M 100 F
Timeline
Italian_cohort
Selection Criteria
- Minimum age
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65
- Maximum age
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79
- Newborns
- Twins
- Countries
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- Italy
- Ethnic Origin
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- Health Status
-
- Other criteria
- Free of major overt chronic diseases for at least 2 years (e.g., cancer, severe organ disease), living independently, and free of dementia
Recruitment
- Sources of recruitment
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- General population
Number of participants
- Number of participants
- 201
- Number of participants with biosamples
Data Collection Event
- Start Date
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2012
- End Date
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2015
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Measurements & methods
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Dietary intake
- yes/no
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- Information collected on habitual food intake collected
- Information collected on type of diet (vegetarian/vegan/omnivore)
- Special dietary aspects collected (e.g. supplements, probiotics, prebiotics and synbiotics)
- Methods
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- Food records
- Other methods
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Food records
- Administration method
- Face-to-face interview
- Administration type
- Paper-based
- Number of recorded days
- 2-7
- Portion size estimation
- Standard portion sizes with aids such as pictures, models, standard household measure, utensils, etc.
- Instrument validity
- No
- Instrument development
- Already existing
- Instrument reference
- Ostan et al., Front Physiol 2018
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Other methods
- Instrument(s) access
- 7-day food record template available
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Alcohol consumption
- Information collected
- Intake
- Questionnaire name/set of questions
- Set of questions
- Administration method
- Face-to-face interview
- Administration type
- Paper-based
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Physical activity
- Information collected
- Scores (ADLs, IADLs, PASE)
- Questionnaire name/set of questions
- Set of questions
- Administration method
- Face-to-face interview
- Domains covered
- Total physical activity
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Sedentary behaviours
- Information collected
- Scores (ADLs, IADLs, PASE)
- Questionnaire name/set of questions
- Set of questions
- Administration method
- Face-to-face interview
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Anthropometric measurements
- Weight/height
- Measured
- Waist/hip circumference
- Measured
- Other measurements
- Body composition (DXA)
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Sociodemographic information
- Information collected
- Sex, age
- Questionnaire name/set of questions
- Set of questions
- Administration method
- Face-to-face interview
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Medication
- Information collected
- Drug name
- Questionnaire name/set of questions
- Set of questions
- Antibiotics
- Yes (recent use of antibiotics was an exclusion criterion)
- Administration method
- Face-to-face interview
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Health status
- Questionnaire name/set of questions
- Blood pressure, hand grip strength, clinical data, family history of diseases, etc.
- Administration method
- Face-to-face interview
- Measurements
- Various
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Biological samples and laboratory measurements
- Samples collected
- Blood, feces
- Number of samples
- 402
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Biomarkers
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Lipids
- Measurement(s)
- Various
- Medium
- Serum
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Glucose
- Measurement(s)
- Various
- Medium
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Inflammation markers
- Measurement(s)
- Various
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Adiposity biomarkers
- Measurement(s)
- Various
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Other biomarkers
- Measurement(s)
- Various
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Metabolomics
- Measurement(s)
- UPLC/Q-TOF-MS
- Medium
- Serum
- Untargeted approach
- yes
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Microbiome
- Microbial material
- 16S rDNA (V3-V4)
- Number of samples/number of individuals
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201
- Library source and library selection
- Metagenome (following the 16S Metagenomic Sequencing Library Preparation protocol by Illumina)
- Medium/tissue
- Faeces
- Sampling collection method
- Sterile collection tube
- Time spent in room temperature
- Few minutes
- Sequencing platform
- Illumina MiSeq
- Read length/depth
- 2x250 bp
- Further details
- True sequencing
- Preservation method
- No, immediately -20°C until delivery to the lab (where stored at -80°C)
- Information on type of meal last eaten prior to sample extraction available
- no