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Elderly microbiome studies, including centenarians and semi-supercentenarians
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Elderly microbiome studies, including centenarians and semi-supercentenarians

Elderly microbiome studies, including centenarians and semi-supercentenarians logo
Networks -
Datasets -
Variables -
Start Year
2007
End Year
2015
Funding
National funds
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Members

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Investigators Contacts
  • Marco Fabbrini
    University of Bologna

Design

Study design
Other

Marker Paper

https://www.sciencedirect.com/science/article/pii/S0960982216303384?via%3Dihub DOI: 10.1016/j.cub.2016.04.016

PUBMED 27185560

Recruitment

Sources of Recruitment
  • Individuals

Number of participants

Number of participants
54
Number of participants with biosamples
Supplementary Information
15 M 39 F

Timeline

elderly-microbiome-studies_-including-centenarians-and-semi-supercentenarians

Selection Criteria
Minimum age
65
Maximum age
109
Newborns
Twins
Countries
  • Italy
International/National/Regional (region name)
Emilia-Romagna region
Ethnic Origin
Health Status

Recruitment

Number of participants

Number of participants
54
Number of participants with biosamples
Data Collection Event
Start Date
2007
End Date
2015

Measurements & methods

Dietary intake

yes/no
  • Information collected on habitual food intake collected
  • Information collected on type of diet (vegetarian/vegan/omnivore)
  • Special dietary aspects collected (e.g. supplements, probiotics, prebiotics and synbiotics)
Methods
  • Food records
Food records
Administration method
Face-to-face interview
Administration type
Paper-based
Number of recorded days
2-7
Instrument validity
No
Instrument development
Already existing

Alcohol consumption

Questionnaire name/set of questions
Set of questions
Administration method
Face-to-face interview
Administration type
Paper-based

Tobacco consumption

Questionnaire name/set of questions
Set of questions
Administration type
Paper-based

Physical activity

Information collected
Scores (ADLs in semi-supercentenarians)
Questionnaire name/set of questions
Set of questions
Administration method
Face-to-face interview
Domains covered
Total physical activity

Sedentary behaviours

Information collected
Scores (ADLs in semi-supercentenarians)
Questionnaire name/set of questions
Set of questions
Administration method
Face-to-face interview

Anthropometric measurements

Weight/height
Measured, for a subset

Sociodemographic information

Information collected
Sex, age
Questionnaire name/set of questions
Set of questions
Administration method
Face-to-face interview

Medication

Information collected
Drug use
Questionnaire name/set of questions
Set of questions
Antibiotics
Last treatment and frequency collected
Administration method
Face-to-face interview

Health status

Information collected
Set of questions
Administration method
Face-to-face interview
Measurements
Various

Biological samples and laboratory measurements

Samples collected
Feces
Number of samples
54

Biomarkers

Inflammation markers

Measurement(s)
Yes, various (only for a subset)
Medium
Plasma

Metabolomics

Measurement(s)
1H-NMR and LC-MS/MS
Medium
Urine (1H-NMR) and serum (LC-MS/MS)
Untargeted approach
yes
Targeted groups
Yes (amino acids, sugars, acyl-carnitines, sphingolipids, glycerophospholipids, eicosanoids)

Microbiome

Microbial material
16S rDNA (V3-V4); DNA
Number of samples/number of individuals
54
Library source and library selection
Metagenome (following the 16S Metagenomic Sequencing Library Preparation protocol by Illumina); shotgun metagenomics (DNA libraries were prepared using the QIAseq FX DNA library kit (Qiagen))
Medium/tissue
Faeces
Sampling collection method
Sterile collection tube
Time spent in room temperature
Few minutes
Sequencing platform
Illumina MiSeq; Illumina NextSeq
Read length/depth
2x250 bp; 2 x 150 bp
Further details
True sequencing (16S rDNA for 54 samples, shotgun metagenomics for 51 samples)
Preservation method
No, immediately -20°C until delivery to the lab (where stored at -80°C)
Information on type of meal last eaten prior to sample extraction available
no