Impact of early life diet on microbiome development & later health
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EarlyMicroHealth project aimed at unravelling the process of intestinal microbiota establishment in infants and to deduce ways to positively modulate this process.This was done by a longitudinal cohort study, evaluating factors, such as gestational age, antibiotics exposure, etc., that negatively impact early-life microbiota development and the impact of diet and nutrition during the first years of life in driving this process. In a second step the impact of interventions, such as omega three fatty acids or probiotics, in minimizing the impact of these factors was assessed. To achieve this aim we formed a multidisciplinary consortium comprising 5 partners from 4 European countries representing: microbiologists, nutritionists, neonatologists, paediatricians, experts in OMICs and Next-Generation Sequencing and bio-informaticians. The exhaustive analyses of the infant microbiome (including phageome, resistome, etc.) and its combination and integration with clinical and nutritional data allowed determining the impact of different early factors on the microbiota development. The knowledge and tools generated within the project offer opportunities for a stronger European Research Area and increasing European competitiveness on the development and commercialization of microbiota-modulating products for infancy.
- Start Year
- 2015
- End Year
- 2020
- Funding
- JPI HDHL “Intestinal Microbiomics” (IM2015)
| Investigators | Contacts |
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Design
- Study design
- Cohort
- Follow Up
- Diet and sample collection at 10-15, 30, 60, 90, 180, 365, 730, 1095 days
Marker Paper
Gómez-Martín, M., Saturio, S., Arboleya, S., Herrero-Morín, D., Calzón, M., López, T., González, S., Gueimonde, M., Association between diet and fecal microbiota along the first year of life, Food Research International (2022). https://doi.org/10.1016/j.foodres.2022.111994
PUBMED
Recruitment
- Sources of Recruitment
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- Individuals
Number of participants
- Number of participants
- 120
- Number of participants with biosamples
- Supplementary Information
- 66 M 54 F
Timeline
EarlyMicroHealth population
Selection Criteria
- Minimum age
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0
- Maximum age
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2
- Newborns
- Twins
- Countries
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- Spain
- International/National/Regional (region name)
- Europe
- Ethnic Origin
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- Health Status
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Recruitment
- Sources of recruitment
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- Other : Recruitement in primare care pediatrician and neonatology section from hospital.
Number of participants
- Number of participants
- 120
- Number of participants with biosamples
Data Collection Event
- Start Date
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2015
- End Date
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2020
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Measurements & methods
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Dietary intake
- yes/no
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- Information collected on habitual food intake collected
- Information collected on type of diet (vegetarian/vegan/omnivore)
- Special dietary aspects collected (e.g. supplements, probiotics, prebiotics and synbiotics)
- Methods
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- FFQ
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FFQ
- Questionnaire name/set of questions
- Adapted PANCAKE questionnarie
- Type of questionnaire
- Semi-quantitative
- Administration method
- Self-reported
- Administration type
- Web/computer-based
- Number of food items
- 153 items at 6 mo and 186 from 6-24 mo
- Reference period
- Previous week
- Frequency categories
- Daily, weekly
- Portion size estimation
- Standard portion sizes with aids such as pictures, models, standard household measure, utensils, etc.
- Instrument validity
- 24h dietary record
- Instrument development
- Adapated from PANCAKE questionnarie for european children
- Instrument reference
- https://docs.google.com/forms/d/1h7EogbIHKZCy2hCBDq6fw7FDXSpm1kPMFK2lfZ9ZU5k/edit?usp=forms_home&ths=true; https://docs.google.com/forms/d/1q10KHi35PrdI4AQLrtxP0soaZ8GXupB7_Zb6JMLyIwM/edit?usp=forms_home&ths=true; https://docs.google.com/forms/d/1_6wh71rw9S0Wsk6E5jWxZRuJqGhVeXQRnXIzu06fGAM/edit?usp=forms_home&ths=true
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Anthropometric measurements
- Weight/height
- Measured
- Waist/hip circumference
- No
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Sociodemographic information
- Information collected
- Gender, age, maternal education level,
- Administration method
- Self-reported
- Administration type
- Web/computer-based
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Medication
- Information collected
- Drug dosage and drug name
- Questionnaire name/set of questions
- Last treatment and frequency collected
- Administration method
- Self-reported
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Biological samples and laboratory measurements
- Samples collected
- Feces
- Number of samples
- 900
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Biomarkers
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Metabolomics
- Measurement(s)
- GC
- Medium
- Feces
- Untargeted approach
- yes
- Targeted groups
- SCFA
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Genomics
- Number of samples/number of individuals
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900
- Extraction protocol/ method
- Q Protocol
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Microbiome
- Microbial material
- DNA
- Number of samples/number of individuals
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900
- Library source and library selection
- 16s Profiling
- Medium/tissue
- Feces
- Time spent in room temperature
- 10 min
- Sequencing platform
- MISeq
- Read length/depth
- Average 177bp and 60000 reads/sample
- Further details
- qPCR of specific intestinal microbial groups
- Preservation method
- Freezing
- Preservation buffer
- no
- Information on type of meal last eaten prior to sample extraction available
- yes