Developmental origins of metabolic diseases: Pisa cohort
Networks
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Datasets
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Variables
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To identify early-life predictive factors of neuro, cardiometabolic development, and their relation to microbiota and metabolome
- Start Year
- 2011
- Funding
- EU FP7, Flagship-project CNR-MIUR InterOmics, JPI-HDHL-INTIMIC, MIUR, Mipaaft
Visit DORIAN-PISAC
Investigators | Contacts |
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Design
- Study design
- Cohort
- Follow Up
- The study plan is to follow offspring from birth to aduthood. So far, the last data collection was carried out at age 5 years
Marker Paper
Guzzardi MA, Ederveen THA, Rizzo F, Weisz A, Collado MC, Muratori F, Gross G, Alkema W, Iozzo P. Maternal pre-pregnancy overweight and neonatal gut bacterial colonization are associated with cognitive development and gut microbiota composition in pre-school-age offspring. Brain Behav Immun. 2022;100:311-320
PUBMED 34920092
Recruitment
- Sources of Recruitment
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- Families
Number of participants
- Number of participants
- 90
- Number of participants with biosamples
Timeline
DORIAN-PISAC
Selection Criteria
- Minimum age
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0
- Pregnant women
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- First Trimester
- Second Trimester
- Third Trimester
- Newborns
- Twins
- Countries
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- Italy
- Territory
- Tuscany
- Ethnic Origin
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- Health Status
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- General population
- Supplementary Information
- General population, no cutoffs, only exclusion of some conditions (e.g. alcohol, smoking, illness, similar)
Recruitment
- Sources of recruitment
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- General population
- General Population
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- Volunteer enrolment
Number of participants
- Number of participants
- 90
- Number of participants with biosamples
Data Collection Event
- Start Date
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2011
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Measurements & methods
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Dietary intake
- yes/no
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- Information collected on habitual food intake collected
- Information collected on type of diet (vegetarian/vegan/omnivore)
- Special dietary aspects collected (e.g. supplements, probiotics, prebiotics and synbiotics)
- Methods
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- 24-hour dietary recall
- FFQ
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24-hour dietary recall
- Administration method
- Phone interview
- Administration type
- Paper-based
- Number of recalls
- 2-7
- Portion size estimation
- Standard portion sizes without aids
- Instrument development
- Metadieta software was used to estimate nutrients
- Instrument reference
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FFQ
- Questionnaire name/set of questions
- FFQ
- Type of questionnaire
- Semi-quantitative
- Administration method
- Self-reported
- Administration type
- Paper-based
- Number of food items
- 61
- Reference period
- varying
- Frequency categories
- varying
- Portion size estimation
- Standard portion sizes without aids
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Alcohol consumption
- Questionnaire name/set of questions
- Set of questions
- Administration method
- Self-reported
- Administration type
- Paper-based
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Tobacco consumption
- Information collected
- Smoker Yes/No
- Questionnaire name/set of questions
- Set of questions
- Administration method
- Self-reported
- Administration type
- Paper-based
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Physical activity
- Information collected
- Frequency / level
- Questionnaire name/set of questions
- Set of questions
- Administration method
- Self-reported
- Domains covered
- Physical activity, occupation
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Anthropometric measurements
- Weight/height
- Measured
- Waist/hip circumference
- Measured
- Other measurements
- Cranial circumference in chidlren
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Sociodemographic information
- Information collected
- Sex, age, residence, marital status, occupation
- Questionnaire name/set of questions
- Set of questions
- Administration method
- Face-to-face interview
- Administration type
- Paper-based
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Medication
- Information collected
- Drug type, especially frequency of antibiotics and corticostroids in infants
- Questionnaire name/set of questions
- Set of questions and medical records
- Antibiotics
- Last treatment and frequency collected
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Health status
- Information collected
- Various at different children's ages, and some also in parents related to cognition, metabolic health, cardiac health, mental health (stress), pregnancy related (diabetes, BP etc)
- Administration type
- Paper-based
- Measurements
- Cognition, parental stress, cardiac function and sizes, blood pressure, OGTT (pregnancy), metabolic and inflammatory markers at birth
- Devices
- Griffith Scale, Raven Scale, Parenting stress scale, EKG, Echocardiography, sphygmomanometer, biochemistry in biofluids
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Biological samples and laboratory measurements
- Samples collected
- Maternal blood, paternal blood, cord-blood, urine, feces, saliva (not all time points)
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Biomarkers
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Lipids
- Measurement(s)
- Various
- Medium
- Plasma, serum
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Glucose
- Measurement(s)
- Various
- Medium
- Plasma, serum
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Inflammation markers
- Measurement(s)
- Various
- Medium
- Serum
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Adiposity biomarkers
- Measurement(s)
- Leptin
- Medium
- Serum
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Other biomarkers
- Measurement(s)
- Other hormones (entero, pancreatic)
- Medium
- Serum
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Metabolomics
- Measurement(s)
- NMR, HP-LC, Mass Spectrometry
- Medium
- Urine, feces
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Microbiome
- Microbial material
- Analysed until 36 months and in parents subgroups, the 60 months ongoing in children (16S V3-V4)
- Library source and library selection
- Medium/tissue
- Feces
- Sampling collection method
- Samples on dry ice
- Time spent in room temperature
- None, one night in fridge
- Sequencing platform
- Illumina Miseq
- Read length/depth
- Preservation method
- Dry ice and -80 within 24h
- Information on type of meal last eaten prior to sample extraction available
- no