Individual Study / DORIAN-PISAC

Developmental origins of metabolic diseases: Pisa cohort

Developmental origins of metabolic diseases: Pisa cohort

DORIAN-PISAC logo
Networks -
Datasets -
Variables -
Start Year
2011
Funding
EU FP7, Flagship-project CNR-MIUR InterOmics, JPI-HDHL-INTIMIC, MIUR, Mipaaft
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Members

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Investigators Contacts
  • Prof. Dr. Patricia Iozzo
    National Research Council (CNR)
  • Dr. Maria Angela Guzzardi
    National Research Council (CNR)
  • Prof. Dr. Patricia Iozzo
    National Research Council (CNR)
  • Dr. Maria Angela Guzzardi
    National Research Council (CNR)

Design

Study design
Cohort
Follow Up
The study plan is to follow offspring from birth to aduthood. So far, the last data collection was carried out at age 5 years

Marker Paper

Guzzardi MA, Ederveen THA, Rizzo F, Weisz A, Collado MC, Muratori F, Gross G, Alkema W, Iozzo P. Maternal pre-pregnancy overweight and neonatal gut bacterial colonization are associated with cognitive development and gut microbiota composition in pre-school-age offspring. Brain Behav Immun. 2022;100:311-320

PUBMED 34920092

Recruitment

Sources of Recruitment
  • Families

Number of participants

Number of participants
90
Number of participants with biosamples

Timeline

DORIAN-PISAC

Selection Criteria
Minimum age
0
Pregnant women
  • First Trimester
  • Second Trimester
  • Third Trimester
Newborns
Twins
Countries
  • Italy
Territory
Tuscany
Ethnic Origin
Health Status
  • General population
Supplementary Information
General population, no cutoffs, only exclusion of some conditions (e.g. alcohol, smoking, illness, similar)

Recruitment

Sources of recruitment
  • General population
General Population
  • Volunteer enrolment

Number of participants

Number of participants
90
Number of participants with biosamples
Data Collection Event
Start Date
2011

Measurements & methods

Dietary intake

yes/no
  • Information collected on habitual food intake collected
  • Information collected on type of diet (vegetarian/vegan/omnivore)
  • Special dietary aspects collected (e.g. supplements, probiotics, prebiotics and synbiotics)
Methods
  • 24-hour dietary recall
  • FFQ
24-hour dietary recall
Administration method
Phone interview
Administration type
Paper-based
Number of recalls
2-7
Portion size estimation
Standard portion sizes without aids
Instrument development
Metadieta software was used to estimate nutrients
Instrument reference
FFQ
Questionnaire name/set of questions
FFQ
Type of questionnaire
Semi-quantitative
Administration method
Self-reported
Administration type
Paper-based
Number of food items
61
Reference period
varying
Frequency categories
varying
Portion size estimation
Standard portion sizes without aids

Alcohol consumption

Questionnaire name/set of questions
Set of questions
Administration method
Self-reported
Administration type
Paper-based

Tobacco consumption

Information collected
Smoker Yes/No
Questionnaire name/set of questions
Set of questions
Administration method
Self-reported
Administration type
Paper-based

Physical activity

Information collected
Frequency / level
Questionnaire name/set of questions
Set of questions
Administration method
Self-reported
Domains covered
Physical activity, occupation

Anthropometric measurements

Weight/height
Measured
Waist/hip circumference
Measured
Other measurements
Cranial circumference in chidlren

Sociodemographic information

Information collected
Sex, age, residence, marital status, occupation
Questionnaire name/set of questions
Set of questions
Administration method
Face-to-face interview
Administration type
Paper-based

Medication

Information collected
Drug type, especially frequency of antibiotics and corticostroids in infants
Questionnaire name/set of questions
Set of questions and medical records
Antibiotics
Last treatment and frequency collected

Health status

Information collected
Various at different children's ages, and some also in parents related to cognition, metabolic health, cardiac health, mental health (stress), pregnancy related (diabetes, BP etc)
Administration type
Paper-based
Measurements
Cognition, parental stress, cardiac function and sizes, blood pressure, OGTT (pregnancy), metabolic and inflammatory markers at birth
Devices
Griffith Scale, Raven Scale, Parenting stress scale, EKG, Echocardiography, sphygmomanometer, biochemistry in biofluids

Biological samples and laboratory measurements

Samples collected
Maternal blood, paternal blood, cord-blood, urine, feces, saliva (not all time points)

Biomarkers

Lipids

Measurement(s)
Various
Medium
Plasma, serum

Glucose

Measurement(s)
Various
Medium
Plasma, serum

Inflammation markers

Measurement(s)
Various
Medium
Serum

Adiposity biomarkers

Measurement(s)
Leptin
Medium
Serum

Other biomarkers

Measurement(s)
Other hormones (entero, pancreatic)
Medium
Serum

Metabolomics

Measurement(s)
NMR, HP-LC, Mass Spectrometry
Medium
Urine, feces

Microbiome

Microbial material
Analysed until 36 months and in parents subgroups, the 60 months ongoing in children (16S V3-V4)
Library source and library selection
Medium/tissue
Feces
Sampling collection method
Samples on dry ice
Time spent in room temperature
None, one night in fridge
Sequencing platform
Illumina Miseq
Read length/depth
Preservation method
Dry ice and -80 within 24h
Information on type of meal last eaten prior to sample extraction available
no