Diet4MicroGut_PRIN2011
Networks
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Datasets
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Variables
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Differences in gut microbiome composition in omnivore, vegetarian and vegan diets.
- Start Year
- 2012
- End Year
- 2013
- Funding
- Italian Ministry of University and Research
Visit Diet4MicroGut
| Investigators | Contacts |
|---|---|
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Design
- Study design
- Cohort
Marker Paper
High-level adherence to a Mediterranean diet beneficially impacts the gut microbiota and associated metabolome. Gut, 2016, 65(11):1812-1821. doi: 10.1136/gutjnl-2015-309957.
PUBMED 26416813
Recruitment
- Sources of Recruitment
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- Individuals
Number of participants
- Number of participants
- 153
- Number of participants with biosamples
Timeline
diet4microgut
Selection Criteria
- Minimum age
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18
- Maximum age
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60
- Newborns
- Twins
- Countries
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- Italy
- International/National/Regional (region name)
- 4 cities in Southern and Northern Italy
- Ethnic Origin
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- Health Status
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- healthy
Recruitment
- Sources of recruitment
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- General population
- Other : recruitment through advertisement, exclusion criteria applied
Number of participants
- Number of participants
- 153
- Number of participants with biosamples
Data Collection Event
- Start Date
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2012
- End Date
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2013
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Measurements & methods
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Dietary intake
- yes/no
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- Information collected on habitual food intake collected
- Information collected on type of diet (vegetarian/vegan/omnivore)
- Special dietary aspects collected (e.g. supplements, probiotics, prebiotics and synbiotics)
- Methods
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- Food records
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Food records
- Administration type
- Paper-based
- Number of recorded days
- 2-7
- Portion size estimation
- Not quantified
- Instrument validity
- No
- Instrument development
- Already existing instrument - self-completed 7-days food diary
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Alcohol consumption
- Questionnaire name/set of questions
- Set of questions
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Tobacco consumption
- Questionnaire name/set of questions
- Set of questions
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Sedentary behaviours
- Questionnaire name/set of questions
- Set of questions
- Administration method
- Self-reported
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Anthropometric measurements
- Weight/height
- Self-reported
- Other measurements
- Weight, height, BMI
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Sociodemographic information
- Information collected
- Sex, age
- Questionnaire name/set of questions
- Set of questions
- Administration method
- Self-reported
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Medication
- Information collected
- Drug dosage and drug name
- Questionnaire name/set of questions
- Set of questions
- Antibiotics
- Yes (antibiotics in the previous 3 months were considered an exclusion factor)
- Administration method
- Self-reported
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Health status
- Information collected
- Gastrointestinal diseases, metabolic diseases, cancer, allergies, cardiovascular diseases, neurodegenerative diseases, constipation, arthritis, pregnancy, breastfeeding were exclusion factors
- Questionnaire name/set of questions
- Set of questions
- Administration method
- Self-reported
- Measurements
- No
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Biological samples and laboratory measurements
- Samples collected
- Urine, saliva, stool
- Number of samples
- 459
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Biomarkers
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Metabolomics
- Measurement(s)
- NMR, GC-MS
- Medium
- Feces, urine
- Untargeted approach
- yes
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Proteomics
- Measurement(s)
- YES on a subset
- Medium
- Feces
- Untargeted approach
- no
- Targeted protein groups
- No
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Genomics
- DNA available
- yes
- Number of samples/number of individuals
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80
- Extraction protocol/ method
- Powe Soil kit (qiagen)
- Genotyping
- No
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Microbiome
- Microbial material
- 16S rRNA V1-V3 regions (454 reads) on 153 subjects, Illumina shotgun on a subset of 50 subjects
- Number of samples/number of individuals
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153
- Library source and library selection
- Metagenome (16S and shotgun), fecal and urinary metabolome, fecal proteome only on a subset
- Medium/tissue
- Feces, urine
- Sampling collection method
- Samples self-collected and transported refrigerated to the laboratory within 24 h, then stored at -80°C
- Time spent in room temperature
- None
- Sequencing platform
- 454 Junior (16S seq), Illumina NextSeq (shotgun)
- Read length/depth
- ~500bp, about 4000 reads/sample (16S seq); about 50M/sample, 2x150bp (shotgun)
- Further details
- Shotgun metagenome; 16S rRNA sequencing
- Preservation method
- Samples self-collected and transported refrigerated to the laboratory within 24 h, then stored at -80°C
- Information on type of meal last eaten prior to sample extraction available
- no